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px330 puro backbone  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
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  • 99

    Structured Review

    New England Biolabs px330 puro backbone
    Px330 Puro Backbone, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 660 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px330+puro+backbone/BbsI-HF/pmc10242444__mmc5-300-5-23
    Average 99 stars, based on 660 article reviews
    px330 puro backbone - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cloning:

    Article Title: Multi-omics analysis reveals distinct non-reversion mechanisms of PARPi resistance in BRCA1- versus BRCA2-deficient mammary tumors
    Article Snippet: Cloning of sgRNAs into the lentiCRISPR v2 backbone was carried out by melting the custom DNA oligos (Microsynth) at 95 C for 5 min, followed by annealing at RT for 2h and subsequently ligation with T4 ligase (NEB) into the BsmBI-digested (Fermantas) backbone. .. Cloning of sgRNAs into the pX330.puro backbone was performed similarly by ligating the previously annealed oligos into the BbsI-HF-digested backbone with T4 ligase (NEB). ..

    Article Title: Multi-omics analysis reveals distinct non-reversion mechanisms of PARPi resistance in BRCA1- versus BRCA2-deficient mammary tumors
    Article Snippet: Cloning of sgRNAs into the lentiCRISPR v2 backbone was carried out by melting the custom DNA oligos (Microsynth) at 95°C for 5 min, followed by annealing at RT for 2h and subsequently ligation with T4 ligase (NEB) into the BsmBI-digested (Fermantas) backbone. .. Cloning of sgRNAs into the pX330.puro backbone was performed similarly by ligating the previously annealed oligos into the BbsI-HF-digested backbone with T4 ligase (NEB). .. KB2P tumor-derived cells were transduced with the cloned lentiCRISPR v2 constructs and KB1P cells were transfected with the generated pX330.puro plasmids using a transfection reagent (TransIT-LT1 from Mirus) following the manufacturer’s protocol.

    Article Title: Multi-omics analysis reveals distinct non-reversion mechanisms of PARPi resistance in BRCA1- versus BRCA2-deficient mammary tumors.
    Article Snippet: Cloning of sgRNAs into the lentiCRISPR v2 backbone was carried out by melting the custom DNA oligos (Microsynth) at 95 C for 5 min, followed by annealing at RT for 2h and subsequently ligation with T4 ligase (NEB) into the BsmBI-digested (Fermantas) backbone. .. Cloning of sgRNAs into the pX330.puro backbone was performed similarly by ligating the previously annealed oligos into the BbsI-HF-digested backbone with T4 ligase (NEB). ..



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